Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Sep-Oct;34(9-10):742-6.
doi: 10.1515/znc-1979-9-1015.

Inhibition of E. coli L-Asparaginase by reaction with 2,3-butanedione. Chemical modification of arginine and histidine residues

D Petz, H G Löffler, F Schneider

Inhibition of E. coli L-Asparaginase by reaction with 2,3-butanedione. Chemical modification of arginine and histidine residues

D Petz et al. Z Naturforsch C Biosci. 1979 Sep-Oct.

Abstract

The inactivation of E. coli asparaginase by 2,3-butanedione studied with L-asparagine and diazooxonorvaline as substrates obeys pseudo first order kinetics. Activity losses are linear with respect to arginine and histidine modification, with complete inactivation being correlated with alteration of one arginine and one histidine per subunit. The rate of inactivation of the enzyme was reduced in the presence of competitive inhibitors like L-2-amino-2-carboxyethane-sulfonamide. Under comparable conditions 1,2-cyclo hexanedione does not affect the activity of L-asparaginase.

PubMed Disclaimer