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. 2005 Aug;43(8):4178-82.
doi: 10.1128/JCM.43.8.4178-4182.2005.

Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates

Laure Diancourt  1 , Virginie Passet, Jan Verhoef, Patrick A D Grimont, Sylvain Brisse
Affiliations

Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates

Laure Diancourt et al. J Clin Microbiol. 2005 Aug.

Abstract

A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyping and showed high (96%) discriminatory power. The MLST approach provides unambiguous data useful for the epidemiology of K. pneumoniae isolates.

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Figures

FIG. 1.
FIG. 1.
Allelic profiles, ribotype patterns, sequence type (ST), ribotype (RT), source information, and MIC of ceftazidime (CAZ) and ciprofloxacin (CIP) of the 67 K. pneumoniae strains. The two first letters of the original strain name correspond to the code of the source medical center. Clinical sources were blood, respiratory tract (RTI), urinary tract (UTI), and soft tissue infections. Epidemiological clusters initially suspected on the basis of the medical center and susceptibility to CAZ (resistance breakpoint, 64 mg/liter) and to CIP (resistance breakpoint, 2 mg/liter) are indicated in the last column. All clinical strains were collected between 1997 and 2001. The UPGMA dendrogram was built from the pairwise distances between allelic profiles using BioNumerics.
See this image and copyright information in PMC

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