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. 2005:25:123-8.

In vivo longitudinal assessment of bone resorption in a fibular osteotomy model using micro-computed tomography

Kim A Powell  1 , Larry Latson, Michael O Ibiwoye, Alan Wolfman, Mark D Grabiner, Maciej Zborowski, Yoshitada Sakai, Ronald J Midura
Affiliations

In vivo longitudinal assessment of bone resorption in a fibular osteotomy model using micro-computed tomography

Kim A Powell et al. Iowa Orthop J. 2005.

Abstract

Longitudinal in vivo micro-computerized tomography (CT) imaging was used to monitor bone resorption in a rat fibula osteotomy model. Quantitative image post-processing techniques were developed for spatially aligning the longitudinal data sets. Nominal length and bone volume in the proximal and distal segments of the fibula after the osteotomy were measured, and quantitative comparisons of bone loss over a 13-week period post-surgery were made in five individual rats. A significant decrease in nominal length and bone volume of the distal segment was observed 13 weeks post surgery. A significant decrease in bone volume was also observed in the proximal segment. However, no change in nominal length was observed for the proximal segment of the fibula. This study illustrates the power of this non-invasive technology to measure in vivo small changes in bone length and volume using just a small cohort of animals.

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Figures

Figure 1
Figure 1
Photograph of the rat mounted on the X-Y-Z rotating platform stage with hind limbs secured and raised into the X-ray source beam. The X-ray source is on the left side of the photograph and the detector and CCD camera are located on the right side of the photograph. The inset figure represents a magnified view looking toward the X-ray source and illustrates the complete shielding of the rodent's body from the X-ray source.
A
A
Posterior view of the hind limbs showing the fibula osteotomies.
B
B
Enlarged views of the left (on left side), and right legs (on right).
A
A
Three-dimensional reconstruction of left proximal tibia and fibula from animal number three taken three weeks post surgery.
B
B
Outer outlines of segmented left proximal tibia and fibula from three weeks (grey) and 13 weeks (black) post surgery. These data sets are shown before and after spatial registration to one another.
A
A
Three-dimensional reconstruction of left distal tibia and fibula from animal number three taken three weeks post surgery.
B
B
Outer outlines of segmented left distal tibia and fibula from three weeks (grey) and 13 weeks (black) post-surgery. These data sets are shown before and after spatial registration to one another.
Figure 5
Figure 5
Plots of cross-sectional area versus length for three, five, ten and 13 weeks post surgery for animal number three. Staring point (nominal length=0) is located at the site of the osteotomy for both the proximal and distal end. The nominal length is oriented along the principal axis of each segment.
Figure 6
Figure 6
Digital images of hematoxylin- and eosin-stained tissue sections of proximal and distal segments of fibula from animal number one. These sections were cut longitudinally along the mid-diathesis of the fibula. The nominal lengths of these bone segments are shown as black lines with double arrows.
See this image and copyright information in PMC

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