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. 2005 Aug 14;11(30):4618-22.
doi: 10.3748/wjg.v11.i30.4618.

Mutation of DNA polymerase beta in esophageal carcinoma of different regions

Guo-Qiang Zhao  1 , Tao Wang, Qin Zhao, Hong-Yan Yang, Xiao-Hui Tan, Zi-Ming Dong
Affiliations

Mutation of DNA polymerase beta in esophageal carcinoma of different regions

Guo-Qiang Zhao et al. World J Gastroenterol. .

Abstract

Aim: To observe the variation of DNA polymerase beta (polbeta) in esophageal carcinoma.

Methods: Thirty specimens containing adjacent normal epithelial tissues were collected from patients in Linzhou region (a high risk area for esophageal squamous carcinoma) and 25 specimens were from a non-high risk area. Total RNA was extracted from the samples and reverse transcription polymerase chain reaction (RT-PCR) was performed. PCR products were cloned and sequenced to investigate the polbeta gene with DNASIS and OMIGA. Statistical significance was evaluated using the chi(2) test.

Results: High-incidence area group: polbeta gene variation was detected in 13 of 30 esophageal carcinoma tissue specimens, and only one variation was found in 30 corresponding adjacent normal tissue specimens. Non high-incidence area group: polbeta gene variation was detected in 5 of 25 esophageal carcinoma tissue specimens, and no variation was found in 25 corresponding adjacent normal tissue specimens. The incidence of polbeta gene variation observed in the high-incidence area group was significantly higher than in the non-high incidence area group. Two mutation hot spots (454-466 and 648-670 nt) and a 58 bp deletion (177-234 nt) were found.

Conclusion: Variations of polbeta perform different functions between the high-incidence areas and the other areas, and may play a more important role in the high-incidence areas.

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Figures

Figure 1
Figure 1
RT-PCR amplification of polβ gene. Lanes 1, 2 and 7: Normal size of PCR products of H1, H2 and H3; lanes 3-6: shorter size of PCR product of H5, H8, H10, and H16; M: DNA marker (from top to bottom: 1 000, 900, 800, 700, 600, 500, 400, 300, 200, 100 bp).
Figure 2
Figure 2
Mutations in the polβ gene. A: 660 nt A→G mutation in H4 carcinoma; B: 670 nt A→G mutation in N1 carcinoma; C: 613 nt A→T mutation in H28 carcinoma.
Figure 3
Figure 3
Comparison between wild type polβ gene fragment and six gene fragments with deletion (177→234 nt).
See this image and copyright information in PMC

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