Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Aug 21;11(31):4916-7.
doi: 10.3748/wjg.v11.i31.4916.

Expression of heparanase mRNA in anti-sense oligonucleotide-transfected human esophageal cancer EC9706 cells

Kui-Sheng Chen  1 , Lan Zhang, Lin Tang, Yun-Han Zhang, Dong-Ling Gao, Liang Yan, Lei Zhang
Affiliations

Expression of heparanase mRNA in anti-sense oligonucleotide-transfected human esophageal cancer EC9706 cells

Kui-Sheng Chen et al. World J Gastroenterol. .

Abstract

Aim: To investigate the effects of anti-sense oligonucleotides (ASODNs) on mRNA expression of heparanase in human esophageal cancer EC9706 cells.

Methods: One non-sense oligonucleotide (N-ODN) and five ASODNs against different heparanase mRNA sites were transfected into EC9706 cells, then the expression of heparanase mRNA in EC9706 cells was studied by in situ hybridization.

Results: The expression of heparanase mRNA could be inhibited by ASODNs. There was no significant difference among five ASODNs (P>0.05), but there was a significant difference between ASODNs and N-ODN or non-transfected group (ASODN1: 2.25+/-0.25, ASODN2: 2.21+/-0.23, ASODN3: 2.23+/-0.23, ASODN4: 2.25+/-0.24 vs N-ODN: 3.47+/-2.80 or non- transfected group: 3.51+/-2.93 respectively, P<0.05).

Conclusion: The expression of heparanase mRNA in EC9706 cells can be inhibited by ASODNs in vivo, and heparanase ASODNs can inhibit metastasis of esophageal squamous cell carcinoma or other tumors by inhibiting the expression of heparanase.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Expression of heparanase mRNA in human esophageal cancer EC9706 cells. A: non- transfected group; B: ASODNs-transfected group; C: control group without probe.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Vlodavsky I, Friedmann Y, Elkin M, Aingorn H, Atzmon R, Ishai-Michaeli R, Bitan M, Pappo O, Peretz T, Michal I, et al. Mammalian heparanase: gene cloning, expression and function in tumor progression and metastasis. Nat Med. 1999;5:793–802. - PubMed
    1. Nakajima M, Irimura T, Di Ferrante D, Di Ferrante N, Nicolson GL. Heparan sulfate degradation: relation to tumor invasive and metastatic properties of mouse B16 melanoma sublines. Science. 1983;220:611–613. - PubMed
    1. Chen KS, Zheng NG, Wu JL, Ding Y, Wang YL. Effects of 8-Br-cAMP on growth related gene expression of human esophageal cancer cell line Eca-109. Jiepou Xuebao. 1999;30:227–229.
    1. Vaday GG, Lider O. Extracellular matrix moieties, cytokines, and enzymes: dynamic effects on immune cell behavior and inflammation. J Leukoc Biol. 2000;67:149–159. - PubMed
    1. Graham LD, Underwood PA. Comparison of the heparanase enzymes from mouse melanoma cells, mouse macrophages, and human platelets. Biochem Mol Biol Int. 1996;39:563–571. - PubMed