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. 2006 Mar;64(3):411-5.
doi: 10.1016/j.mimet.2005.06.002. Epub 2005 Aug 10.

Surface plasmon resonance for real-time evaluation of immobilized fructosyltransferase activity

Moshe Shemesh  1 , Doron Steinberg
Affiliations

Surface plasmon resonance for real-time evaluation of immobilized fructosyltransferase activity

Moshe Shemesh et al. J Microbiol Methods. 2006 Mar.

Abstract

The extracellular enzyme fructosyltransferase (FTF) is considered to be a significant virulence factor in the dental biofilm. We have developed a method using surface plasmon resonance to detect the activity of immobilized FTF in situ. This real time technique provides a sensitive direct assay for characterizing functional properties of immobilized enzymes such as FTF.

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