Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005;41(3):262-8.
doi: 10.1111/j.1472-765X.2005.01749.x.

Effect of media, temperature and culture conditions on the species population and antibiotic resistance of enterococci from broiler chickens

C R Jackson  1 , P J Fedorka-Cray, M C Jackson-Hall, L M Hiott
Affiliations

Effect of media, temperature and culture conditions on the species population and antibiotic resistance of enterococci from broiler chickens

C R Jackson et al. Lett Appl Microbiol. 2005.

Abstract

Aims: The effect of media type, incubation temperature and enrichment period on the species population and antibiotic susceptibility of enterococci from poultry carcass rinsates was determined.

Methods and results: Aliquots of rinsates, incubated in BBL Enterococcosel broth at 37 degrees C, 42 degrees C, or 45 degrees C for 24 and 48 h, were inoculated onto BBL Enterococcosel and M-enterococcus agar. Presumptive positive colonies were identified to species and tested for antibiotic resistance. Significant differences (P < or = 0.05) were observed for media and temperature. More Enterococcus faecalis were isolated from M-enterococcus media and at 37 degrees C while more E. faecium were isolated from Enterococcosel agar and at 45 degrees C. The number of antibiotic-resistant E. faecalis and E. faecium were also affected by media and temperature.

Conclusions: Culture conditions for enterococci affect the observed species and antibiotic resistance patterns and therefore should be carefully considered.

Significance and impact of the study: This study indicates that media and temperature can influence the recovery and selection of enterococcal species and antibiotic susceptibility.

PubMed Disclaimer