Functional changes in rod and cone pathways after photoreceptor loss in light-damaged rats
- PMID: 16109651
- DOI: 10.1080/02713680590968592
Functional changes in rod and cone pathways after photoreceptor loss in light-damaged rats
Abstract
Purpose: To determine the functional changes in the rod and cone pathways after photoreceptor loss by continuous light exposure.
Methods: Fifty-four male Sprague-Dawley rats were exposed to diffuse fluorescent light of 2000 lux for 24 or 48 hr. Two weeks after the light exposure, full-field scotopic and photopic electroretinograms (ERGs) were elicited by different stimulus intensities with a maximum luminance of 0.84 log cd-s/m2. The amplitudes of the a- and b-waves of the scotopic ERGs and the b-wave of the photopic ERGs were measured. The animals were sacrificed after the ERG recordings, and the number of surviving rod and cone nuclei in the outer nuclear layer was counted.
Results: The logarithm (log) of the amplitudes of the maximum rod a-wave (rod Va(max)) and b-wave (rod Vb(max)) was reduced monotonically with a decrease in the rod nucleus counts (p < 0.0001). The regression line for the rod Va(max) decrease was significantly steeper than that for the rod Vb(max) (p < 0.005). The maximum b-wave amplitudes of the photopic ERGs (cone Vb(max)) were significantly correlated with the number of cone nuclei in a log-linear fashion. The slopes of the regression lines for the rod Vb(max) and cone Vb(max) were 0.0067 and 0.0140, respectively, which indicates that the amplitude of the cone b-wave was more severely affected than that of the rod b-waves by light-induced photoreceptor degeneration (p < 0.005).
Conclusions: The amplitudes of the rod and cone ERGs were correlated with rod and cone nuclei counts in a log-linear fashion in light-damaged rats. The functional loss from the photoreceptor death had a greater effect on the cone pathway than on the rod pathway when the retinal function was assessed by the b-wave.
Similar articles
-
Quantitative relationship of the scotopic and photopic ERG to photoreceptor cell loss in light damaged rats.Exp Eye Res. 2000 May;70(5):693-705. doi: 10.1006/exer.2000.0842. Exp Eye Res. 2000. PMID: 10870528
-
The status of cones in the rhodopsin mutant P23H-3 retina: light-regulated damage and repair in parallel with rods.Invest Ophthalmol Vis Sci. 2008 Mar;49(3):1116-25. doi: 10.1167/iovs.07-1158. Invest Ophthalmol Vis Sci. 2008. PMID: 18326739
-
Cone-rod dependence in the rat retina: variation with the rate of rod damage.Invest Ophthalmol Vis Sci. 2009 Jun;50(6):3017-23. doi: 10.1167/iovs.08-3004. Epub 2009 Jan 31. Invest Ophthalmol Vis Sci. 2009. PMID: 19182251
-
The dystrophic retina in multisystem disorders: the electroretinogram in neuronal ceroid lipofuscinoses.Eye (Lond). 1998;12 ( Pt 3b):580-90. doi: 10.1038/eye.1998.148. Eye (Lond). 1998. PMID: 9775220 Review.
-
[Indications for electrophysiologic studies of the eye].Klin Monbl Augenheilkd. 1989 May;194(5):333-6. doi: 10.1055/s-2008-1046379. Klin Monbl Augenheilkd. 1989. PMID: 2664323 Review. German.
Cited by
-
Transplantation of human embryonic stem cell-derived photoreceptors restores some visual function in Crx-deficient mice.Cell Stem Cell. 2009 Jan 9;4(1):73-9. doi: 10.1016/j.stem.2008.10.015. Cell Stem Cell. 2009. PMID: 19128794 Free PMC article.
-
Phototoxic effects of commercial photographic flash lamp on rat eyes.Doc Ophthalmol. 2006 Nov;113(3):155-64. doi: 10.1007/s10633-006-9024-y. Epub 2006 Oct 3. Doc Ophthalmol. 2006. PMID: 17016736
-
The Effect of intravitreal N-methyl-DL-aspartic acid on the electroretinogram in Royal College of surgeons rats.Jpn J Ophthalmol. 2007 May-Jun;51(3):165-74. doi: 10.1007/s10384-007-0420-y. Epub 2007 Jun 7. Jpn J Ophthalmol. 2007. PMID: 17554477
-
Retinal compensatory changes after light damage in albino mice.Mol Vis. 2012;18:675-93. Epub 2012 Mar 24. Mol Vis. 2012. PMID: 22509098 Free PMC article.
-
Contribution of N-methyl-DL-aspartic acid (NMDA)-sensitive neurons to generating oscillatory potentials in Royal College of Surgeons rats.Doc Ophthalmol. 2013 Oct;127(2):131-40. doi: 10.1007/s10633-013-9394-x. Epub 2013 Jun 7. Doc Ophthalmol. 2013. PMID: 23744447
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources