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. 2005 Sep;187(17):6231-7.
doi: 10.1128/JB.187.17.6231-6237.2005.

Xanthomonas campestris pv. campestris possesses a single gluconeogenic pathway that is required for virulence

Dong-Jie Tang  1 , Yong-Qiang He, Jia-Xun Feng, Bao-Ren He, Bo-Le Jiang, Guang-Tao Lu, Baoshan Chen, Ji-Liang Tang
Affiliations

Xanthomonas campestris pv. campestris possesses a single gluconeogenic pathway that is required for virulence

Dong-Jie Tang et al. J Bacteriol. 2005 Sep.

Abstract

Disruption of ppsA, a key gene in gluconeogenesis, of Xanthomonas campestris pv. campestris resulted in the failure of the pathogen to grow in medium with pyruvate or C4-dicarboxylates as the sole carbon source and a significant reduction in virulence, indicating that X. campestris pv. campestris possesses only the malic enzyme-PpsA route in gluconeogenesis, which is required for virulence.

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Figures

FIG. 1.
FIG. 1.
Schematic drawing of the gluconeogenic pathway in microorganisms. The PckA route is shown as a blue arrow, the malic enzyme-PpsA route as red arrows, and the glyoxylate cycle as green arrows (modified from reference 24). CoA, coenzyme A.
FIG. 2.
FIG. 2.
Growth of X. campestris pv. campestris strains (▴, wild-type strain 8004; ○, ppsA mutant NK1880; •, ppsA-complemented strain CNK1880) in media with different carbon sources. The NCM medium was supplemented with glucose (A), sucrose (B), fumarate (C), malate (D), succinate (E), or pyruvate (F) at 0.5% or sodium acetate (NaAC) at 0.15% (G). Data presented were from a representative experiment.
FIG. 3.
FIG. 3.
ppsA is required for virulence and in planta growth of X. campestris pv. campestris. (A) Black rot disease symptoms caused by X. campestris pv. campestris strains on inoculated leaves of Chinese radish. Photographs were taken at day 10 postinoculation. (B) Average lesion lengths caused by X. campestris pv. campestris strains. Values are the means ± standard deviations (SD) from three repeats, using two leaves each from 25 plants. (C) Growth of bacteria in inoculated leaves. Data are the means ± SD from three repeats. ▴, wild-type strain 8004; ○, ppsA mutant NK1880; •, ppsA-complemented strain CNK1880.
FIG. 4.
FIG. 4.
The mls mutant and aceA mutant are unable to grow on minimal medium supplemented with acetate as the sole carbon source. ▴, wild-type strain 8004; ⋄, mls mutant NK0216; ⧫, aceA mutant NK0217. OD600, optical density at 600 nm.
FIG. 5.
FIG. 5.
mls and aceA are not required for virulence and in planta growth of X. campestris pv. campestris. (A) Black rot disease symptoms caused by X. campestris pv. campestris strains on inoculated leaves of Chinese radish. Photographs were taken at day 10 postinoculation. (B) Average lesion lengths caused by X. campestris pv. campestris strains. Values are the means ± standard deviations (SD) from three repeats, each with 50 leaves. (C) Growth of bacteria in inoculated leaves. Data are the means ± SD from three repeats. ▴, wild-type strain 8004; ⋄, mls mutant NK0216; ⧫, aceA mutant NK0217.
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