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. 2005 Oct 1;251(1):143-7.
doi: 10.1016/j.femsle.2005.07.036.

A novel neisserial shuttle plasmid: a useful new tool for meningococcal research

Clíona A O' Dwyer  1 , Paul R Langford, J Simon Kroll
Affiliations

A novel neisserial shuttle plasmid: a useful new tool for meningococcal research

Clíona A O' Dwyer et al. FEMS Microbiol Lett. .

Abstract

We report the identification and nucleotide sequence analysis of a cryptic plasmid pMIDG2830 from the Gram-negative bacterium Neisseria flavescens. The largest open reading frame encodes a protein similar to the replication protein, RepA, found in pAB49 from Acinetobacter baumannii and pNI10 from Pseudomonas. Modified by the incorporation of a kanamycin resistance cassette, the plasmid can be stably maintained in Escherichia coli and Neisseria meningitidis, and can be used as a shuttle plasmid in meningococcal research.

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