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. 2005 Aug;162(8):959-62.
doi: 10.1016/j.jplph.2005.01.008.

Induction of multiple shoots from leaf segments, in vitro-flowering and fruiting of a dwarf tomato (Lycopersicon esculentum)

Kokkirala Venugopal Rao  1 , Kasula Kiranmayee, Umate Pavan, Telakalapalli Jaya Sree, Alleni V Rao, Abbagani Sadanandam
Affiliations

Induction of multiple shoots from leaf segments, in vitro-flowering and fruiting of a dwarf tomato (Lycopersicon esculentum)

Kokkirala Venugopal Rao et al. J Plant Physiol. 2005 Aug.

Abstract

Multiple shoots were induced from leaf explants of Lycopersicon esculentum cultivar MicroTom, within 20-25d, on MS medium supplemented with 8.9 microM benzylaminopurine (BAP)+1.14 microM indole-3-acetic acid (IAA). For rooting, elongated microshoots were excised and transferred onto MS medium supplemented with 4.9 microM indole-3-butyric acid (IBA). Well-developed roots and flower raceme were obtained on d 7 and 13, respectively, upon transfer of the microshoots onto rooting medium. The flowers self-fertilized in vitro and produced mature fruits in additional 15-17d of culture.

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