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Clinical Trial
. 2005 Sep;12(9):1127-9.
doi: 10.1128/CDLI.12.9.1127-1129.2005.

Comparison of the antibodies in lymphocyte supernatant and antibody-secreting cell assays for measuring intestinal mucosal immune response to a novel oral typhoid vaccine (M01ZH09)

B D Kirkpatrick  1 , Matthew D Bentley, Anette M Thern, Catherine J Larsson, Cassandra Ventrone, Meera V Sreenivasan, Lou Bourgeois
Affiliations
Clinical Trial

Comparison of the antibodies in lymphocyte supernatant and antibody-secreting cell assays for measuring intestinal mucosal immune response to a novel oral typhoid vaccine (M01ZH09)

B D Kirkpatrick et al. Clin Diagn Lab Immunol. 2005 Sep.

Abstract

Antibody-secreting cell (ASC) and antibodies in lymphocyte supernatant (ALS) assays are used to assess intestinal mucosal responses to enteric infections and vaccines. The ALS assay, performed on cell supernatants, may represent a convenient alternative to the more established ASC assay. The two methods, measuring immunoglobulin A to Salmonella enterica serovar Typhi lipopolysaccharide, were compared in volunteers vaccinated with a live-attenuated typhoid vaccine M01ZH09. The specificity of the ALS assay compared to the ASC assay was excellent (100%), as was sensitivity (82%). The ALS assay was less sensitive than the ASC assay at <or=42 spots/10(6) peripheral blood lymphocytes.

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Figures

FIG. 1.
FIG. 1.
The S. enterica serovar Typhi LPS IgA ASC and ALS assays demonstrate concordance in volunteers vaccinated with oral typhoid vaccine M01ZH09.
See this image and copyright information in PMC

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