Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Jun 11;20(11):2773-6.
doi: 10.1093/nar/20.11.2773.

The influence of single base triplet changes on the stability of a pur.pur.pyr triple helix determined by affinity cleaving

P A Beal  1 , P B Dervan
Affiliations
Free PMC article

The influence of single base triplet changes on the stability of a pur.pur.pyr triple helix determined by affinity cleaving

P A Beal et al. Nucleic Acids Res. .
Free PMC article

Abstract

The influence of sixteen base triplet changes at a single position within a pur.pur.pyr triple helix was examined by affinity cleaving. For the 15 base pair target site studied here, G.GC, A.AT and T.AT triplets stabilize a triple helix to a greater extent than the other 13 natural triplets (pH = 7.4, 25 degrees C). Weaker interactions were detected for the C.AT, A.GC and T.CG triplets. The absence of specific, highly stabilizing interactions between third strand bases and the CG or TA base pairs demonstrates a current sequence limitation to formation of this structure. Models for the two dimensional base triplet interactions for all possible 16 natural triplets are presented.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Biochemistry. 1991 Sep 17;30(37):9022-30 - PubMed
    1. Electrophoresis. 1990 May;11(5):355-60 - PubMed
    1. Biochemistry. 1991 Jun 25;30(25):6081-8 - PubMed
    1. Biochemistry. 1992 Mar 17;31(10):2829-34 - PubMed
    1. Biochemistry. 1992 Jan 21;31(2):320-7 - PubMed

Publication types