Comment
doi: 10.1073/pnas.0506279102.
Epub 2005 Sep 6.
Two ways to trap a gene in mice
Affiliations
- PMID: 16150712
- PMCID: PMC1201614
- DOI: 10.1073/pnas.0506279102
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Comment
Two ways to trap a gene in mice
Proc Natl Acad Sci U S A.
.
No abstract available
Figures
Gene trapping versus targeted trapping. (A) Gene trapping depends on random insertions of a promoterless reporter gene, such as βgeo, equipped with splice acceptor (SA) and polyadenylation (pA) signals. The reporter is activated after insertions into introns of expressed genes to generate a fusion mRNA that can be characterized by 5′ RACE. (B) Targeted trapping relies on homologous recombination to introduce a promoterless gene-trap cassette into predefined loci. The trapping cassette is flanked by genomic sequences of the target locus that do not contain the promoter of the target gene.
Comment on
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Gene targeting using a promoterless gene trap vector ("targeted trapping") is an efficient method to mutate a large fraction of genes.Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13188-93. doi: 10.1073/pnas.0505474102. Epub 2005 Aug 29. Proc Natl Acad Sci U S A. 2005. PMID: 16129827 Free PMC article.
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