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Comment
. 2005 Sep 13;102(37):13001-2.
doi: 10.1073/pnas.0506279102. Epub 2005 Sep 6.

Two ways to trap a gene in mice

William C Skarnes  1
Affiliations
Comment

Two ways to trap a gene in mice

William C Skarnes. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Fig. 1.
Fig. 1.
Gene trapping versus targeted trapping. (A) Gene trapping depends on random insertions of a promoterless reporter gene, such as βgeo, equipped with splice acceptor (SA) and polyadenylation (pA) signals. The reporter is activated after insertions into introns of expressed genes to generate a fusion mRNA that can be characterized by 5′ RACE. (B) Targeted trapping relies on homologous recombination to introduce a promoterless gene-trap cassette into predefined loci. The trapping cassette is flanked by genomic sequences of the target locus that do not contain the promoter of the target gene.
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References

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