Farnesol and geranylgeraniol modulate the structural properties of phosphatidylethanolamine model membranes

Abstract

The biological activity of farnesol (FN) and geranylgeraniol (GG) and their isoprenyl groups is related to membrane-associated processes. We have studied the interactions of FN and GG with 1,2-dielaidoyl-sn-glycero-3-phosphoethanolamine (DEPE) membranes using DSC and X-ray diffraction. Storage of samples at low temperature for a long time favors a multidomain system formed by a lamellar crystalline (Lc) phase and isoprenoids (ISPs) aggregates. We demonstrate that ISPs alter the thermotropic behavior of DEPE, thereby promoting a HII growth in a lamellar Lc phase with a reduced degree of hydration. The HII phase occurs with the same repeat distance (dHII=5.4 nm) as the Lc phase and upon heating it expands considerably (deltad/deltaT approximately 0.22 nm/ degrees C). The dimensional stabilization of this HII phase coincides with the transition temperature of the Lc to Lalpha phase. Thereafter, the system DEPE/ISP will progress by increasing the nonlamellar-forming propensity and reaching a single HII phase at high temperature. The cooling scan followed a similar structural path, except that the system went into a stable gel phase Lbeta with a repeat distance, dLbeta=6.5 nm, in co-existence with a HII phase. The formation of ISP microdomains in model PE membranes substantiates the importance of the isoprenyl group in the binding of isoprenylated proteins to membranes and in lipid-lipid interactions through modulation of the membrane structure.