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. 2005 Oct;34(2):104-7.
doi: 10.1016/j.jcv.2005.02.010.

Sequence variation in primer targets affects the accuracy of viral quantitative PCR

David M Whiley  1 , Theo P Sloots
Affiliations

Sequence variation in primer targets affects the accuracy of viral quantitative PCR

David M Whiley et al. J Clin Virol. 2005 Oct.

Abstract

This study was designed to assess the effect of sequence mismatches between primers and their targets on viral quantitative PCR. Numerous primers were constructed incorporating various mismatches with a target sequence on the BKV T antigen gene. When using these primers in standard Taqman two-step cycling conditions, as few as two mismatches in a single primer increased cycle threshold values and significantly influenced the calculation of viral load.

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