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. 2006 Feb;20(2):195-9.
doi: 10.1002/bmc.552.

Examination of dye-protein interaction by gel-permeation chromatography

Jolanta Sereikaite  1 , Vladas-Algirdas Bumelis
Affiliations

Examination of dye-protein interaction by gel-permeation chromatography

Jolanta Sereikaite et al. Biomed Chromatogr. 2006 Feb.

Abstract

The interaction of Cibacron blue F3G-A with two therapeutic proteins, recombinant human growth hormone and recombinant human interferon-alpha2b, has been examined by applying gel-permeation chromatography in combination with the absorption difference spectroscopy. The complexes of these proteins with Cibacron blue F3G-A have been isolated, and their absorbance spectra have been registered. The influence of Cibacron blue F3G-A on the oligomeric state of proteins has been investigated. It was found that Cibacron blue F3G-A promotes the generation of interferon-alpha2b dimers at pH 5.0.

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