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. 2005 Oct;54(10):1506-8.
doi: 10.1136/gut.2005.072447.

High levels of disease related prion protein in the ileum in variant Creutzfeldt-Jakob disease

S Joiner, J M Linehan, S Brandner, J D F Wadsworth, J Collinge

High levels of disease related prion protein in the ileum in variant Creutzfeldt-Jakob disease

S Joiner et al. Gut. 2005 Oct.
No abstract available

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Figures

Figure 1
Figure 1
(A, B) High sensitivity immunoblots using anti-prion protein (PrP) monoclonal antibody 3F4. (A) Proteinase K digested sodium phosphotungstic acid pellets from 0.5 ml of 10% terminal ileum homogenates from variant Creutzfeldt-Jakob disease (vCJD) patients 1–4 or sporadic CJD (sCJD) patients 1 and 2. (B) Proteinase K digested sodium phosphotungstic acid pellets from 0.5 ml of 10% normal human tonsil homogenate (normal tonsil) or 0.5 ml of 10% normal human tonsil homogenate spiked with 2.5 μl of 10% brain homogenate from vCJD patient No 4 (spiked tonsil) were compared with a proteinase K digested sodium phosphotungstic acid pellet from 0.5 ml of 10% terminal ileum homogenate from the same vCJD patient. (C) Photomicrograph showing abnormal PrP immunoreactivity in a lymphoid follicle in vCJD terminal ileum (anti-PrP monoclonal antibody ICSM 35). Scale bar, 100 μm. Inset, high power magnification of PrP deposits.
See this image and copyright information in PMC

References

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