doi: 10.1016/j.ab.2005.08.013.
Epub 2005 Sep 8.
Composition gradient static light scattering: a new technique for rapid detection and quantitative characterization of reversible macromolecular hetero-associations in solution
Affiliations
- PMID: 16188220
- DOI: 10.1016/j.ab.2005.08.013
Item in Clipboard
Composition gradient static light scattering: a new technique for rapid detection and quantitative characterization of reversible macromolecular hetero-associations in solution
Anal Biochem.
.
Abstract
A method for rapid detection and quantitative characterization of associations between two proteins in solution is presented, based upon continuous measurement of the Rayleigh light scattering and UV absorbance of a solution whose composition is varied with time in a controlled fashion. Collection and subsequent analysis of data are both rapid and semiautomatic. The results of validation experiments demonstrate that the technique can correctly identify complexes and reliably evaluate equilibrium constants for hetero-association.
Similar articles
-
New methods for measuring macromolecular interactions in solution via static light scattering: basic methodology and application to nonassociating and self-associating proteins.Anal Biochem. 2005 Feb 1;337(1):103-10. doi: 10.1016/j.ab.2004.09.045. Anal Biochem. 2005. PMID: 15649382
-
Free-solution, label-free protein-protein interactions characterized by dynamic light scattering.Biophys J. 2010 Jan 20;98(2):297-304. doi: 10.1016/j.bpj.2009.09.061. Biophys J. 2010. PMID: 20338851 Free PMC article.
-
Rapid quantitative characterization of protein interactions by composition gradient static light scattering.Biophys J. 2006 Mar 15;90(6):2164-9. doi: 10.1529/biophysj.105.074310. Epub 2005 Dec 30. Biophys J. 2006. PMID: 16387762 Free PMC article.
-
Static light scattering to characterize membrane proteins in detergent solution.Methods. 2008 Oct;46(2):73-82. doi: 10.1016/j.ymeth.2008.06.012. Epub 2008 Jul 14. Methods. 2008. PMID: 18625320 Review.
-
Quantitative characterization of reversible macromolecular associations via sedimentation equilibrium: an introduction.Exp Mol Med. 2000 Mar 31;32(1):1-5. doi: 10.1038/emm.2000.1. Exp Mol Med. 2000. PMID: 10762054 Review.
Cited by
-
Higher-order oligomerization promotes localization of SPOP to liquid nuclear speckles.EMBO J. 2016 Jun 15;35(12):1254-75. doi: 10.15252/embj.201593169. Epub 2016 May 23. EMBO J. 2016. PMID: 27220849 Free PMC article.
-
pH-dependent self-association of zinc-free insulin characterized by concentration-gradient static light scattering.Biophys Chem. 2010 May;148(1-3):28-33. doi: 10.1016/j.bpc.2010.02.002. Epub 2010 Feb 8. Biophys Chem. 2010. PMID: 20202737 Free PMC article.
-
The analysis of macromolecular interactions by sedimentation equilibrium.Methods. 2011 May;54(1):145-56. doi: 10.1016/j.ymeth.2010.12.005. Epub 2010 Dec 16. Methods. 2011. PMID: 21167941 Free PMC article.
-
Rescue of the temperature-sensitive, autosomal-recessive mutation R298S in the sodium-bicarbonate cotransporter NBCe1-A characterized by a weakened dimer and abnormal aggregation.Biochim Biophys Acta. 2015 Jun;1850(6):1286-96. doi: 10.1016/j.bbagen.2015.02.014. Epub 2015 Mar 3. Biochim Biophys Acta. 2015. PMID: 25743102 Free PMC article.
-
Mg(2+)-linked self-assembly of FtsZ in the presence of GTP or a GTP analogue involves the concerted formation of a narrow size distribution of oligomeric species.Biochemistry. 2012 Jun 5;51(22):4541-50. doi: 10.1021/bi300401b. Epub 2012 May 22. Biochemistry. 2012. PMID: 22568594 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
