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. 2005 Nov 1;346(1):132-8.
doi: 10.1016/j.ab.2005.08.013. Epub 2005 Sep 8.

Composition gradient static light scattering: a new technique for rapid detection and quantitative characterization of reversible macromolecular hetero-associations in solution

Arun K Attri  1 , Allen P Minton
Affiliations

Composition gradient static light scattering: a new technique for rapid detection and quantitative characterization of reversible macromolecular hetero-associations in solution

Arun K Attri et al. Anal Biochem. .

Abstract

A method for rapid detection and quantitative characterization of associations between two proteins in solution is presented, based upon continuous measurement of the Rayleigh light scattering and UV absorbance of a solution whose composition is varied with time in a controlled fashion. Collection and subsequent analysis of data are both rapid and semiautomatic. The results of validation experiments demonstrate that the technique can correctly identify complexes and reliably evaluate equilibrium constants for hetero-association.

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