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. 2005 Sep 1;30(17):2275-7.
doi: 10.1364/ol.30.002275.

Evaluating cutaneous photoaging by use of multiphoton fluorescence and second-harmonic generation microscopy

Sung-Jan Lin  1 , Ruei- Wu Jr, Hsin-Yuan Tan, Wen Lo, Wei-Chou Lin, Tai-Horng Young, Chih-Jung Hsu, Jau-Shiuh Chen, Shiou-Hwa Jee, Chen-Yuan Dong
Affiliations

Evaluating cutaneous photoaging by use of multiphoton fluorescence and second-harmonic generation microscopy

Sung-Jan Lin et al. Opt Lett. .

Abstract

The photoaging process of facial skin is investigated by use of multiphoton fluorescence and second-harmonic generation (SHG) microscopy. We obtain the autofluorescence (AF) and SHG images of the superficial dermis from the facial skin of three patients aged 20, 40, and 70 years. The results show that areas of AF increase with age, whereas areas of SHG decrease with age. The results are consistent with the histological findings in which collagen is progressively replaced by elastic fibers. The AF and SHG changes in photoaging are quantified by a SHG to autofluorescence aging index of dermis (SAAID). Our results suggest that SAAID can be a good indicator of the severity of photoaging.

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