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. 2005 Aug 31;2005(4):221-7.
doi: 10.1155/MI.2005.221.

Ozone therapy on rats submitted to subtotal nephrectomy: role of antioxidant system

José Luis Calunga  1 , Zullyt B Zamora, Aluet Borrego, Sarahí del Río, Ernesto Barber, Silvia Menéndez, Frank Hernández, Teresita Montero, Dunia Taboada
Affiliations

Ozone therapy on rats submitted to subtotal nephrectomy: role of antioxidant system

José Luis Calunga et al. Mediators Inflamm. .

Abstract

Chronic renal failure (CRF) represents a world health problem. Ozone increases the endogenous antioxidant defense system, preserving the cell redox state. The aim of this study is to evaluate the effect of ozone/oxygen mixture in the renal function, morphology, and biochemical parameters, in an experimental model of CRF (subtotal nephrectomy). Ozone/oxygen mixture was applied daily, by rectal insufflation (0.5 mg/kg) for 15 sessions after the nephrectomy. Renal function was evaluated, as well as different biochemical parameters, at the beginning and at the end of the study (10 weeks). Renal plasmatic flow (RPF), glomerular filtration rate (GFR), the urine excretion index, and the sodium and potassium excretions (as a measurement of tubular function) in the ozone group were similar to those in Sham group. Nevertheless, nephrectomized rats without ozone (positive control group) showed the lowest RPF, GFR, and urine excretion figures, as well as tubular function. Animals treated with ozone showed systolic arterial pressure (SAP) figures lower than those in the positive control group, but higher values compared to Sham group. Serum creatinine values and protein excretion in 24 hours in the ozone group were decreased compared with nephrectomized rats, but were still higher than normal values. Histological study demonstrated that animals treated with ozone showed less number of lesions in comparison with nephrectomized rats. Thiobarbituric acid reactive substances were significantly increased in nephrectomized and ozone-treated nephrectomized rats in comparison with Sham group. In the positive control group, superoxide dismutase (SOD) and catalase (CAT) showed the lowest figures in comparison with the other groups. However, ozone/oxygen mixture induced a significant stimulation in the enzymatic activity of CAT, SOD, and glutathione peroxidase, as well as reduced glutathione in relation with Sham and positive control groups. In this animal model of CRF, ozone rectal administrations produced a delay in the advance of the disease, protecting the kidneys against vascular, hemorheological, and oxidative mechanisms. This behavior suggests ozone therapy has a protective effect on renal tissue by downregulation of the oxidative stress shown in CRF.

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Figures

Figure 1
Figure 1
Histological study of the renal cortex. (a) Sham group (200×), normal morphology; (b) positive control group (200×), glomerular collapse, cortical hemorrhages, vascular congestion, dilatation of convoluted tubules and glomerular capsule dilatation; and (c) ozone group (400×), a discrete dilatation of convoluted tubules.
See this image and copyright information in PMC

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