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. 2006 May;65(2):354-6.
doi: 10.1016/j.mimet.2005.08.009. Epub 2005 Sep 27.

Direct detection of human Staphylococcus aureus carriage in the nose using the Lightcycler Staphylococcus kit

Roos S R Choudhurry  1 , Damian C Melles, Kimberly Eadie, Margreet Vos, Heiman F L Wertheim, Henry A Verbrugh, Alex van Belkum, Willem B van Leeuwen
Affiliations

Direct detection of human Staphylococcus aureus carriage in the nose using the Lightcycler Staphylococcus kit

Roos S R Choudhurry et al. J Microbiol Methods. 2006 May.

Abstract

The Lightcycler Staphylococcus kit is a diagnostic tool for direct real-time detection of Staphylococcus aureus in clinical materials. We show here that detection of S. aureus nasal carriage using this test is hampered by competition of DNA from coagulase-negative staphylococci. However the test is well suited for species identification after culture and the identification of high-load S. aureus carriers.

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