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. 1977 Jan-Feb;46(1):169-71.

[Methanol oxidation by the NAD-specific dehydrogenase of methane-assimilating yeasts]

[Article in Russian]
Ia A Simisker, S K Mikelsaar, E H Heinara
  • PMID: 16199

[Methanol oxidation by the NAD-specific dehydrogenase of methane-assimilating yeasts]

[Article in Russian]
Ia A Simisker et al. Mikrobiologiia. 1977 Jan-Feb.

Abstract

The activity of NAD-specific dehydrogenase (NAD-DH) capable of methanol oxidation was found in cell-free extracts of methanol assimilating yeast cultures Pichia pinus and Candida boidinii. The optimum pH of the incubation mixture for the enzyme is 7.8--7.9; the value of Km of the enzyme for methanol is 1.4-10(-4) M in P. pinus and 1.04-10(-3) M in C. boidinii. NAD-DH is absent from the cells cultivated on glucose, and is induced with growth on a medium containing methanol.

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