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. 1992 Jul;12(7):2919-23.
doi: 10.1128/mcb.12.7.2919-2923.1992.

High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors

K R Thomas  1 , C Deng, M R Capecchi
Affiliations

High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors

K R Thomas et al. Mol Cell Biol. 1992 Jul.

Abstract

Mutations were targeted to the Hprt locus in murine embryonic stem cells by using sequence replacement vectors. When the vector was designed such that the mutated sequences were flanked on both sides by several kilobases of DNA homologous to the target locus, replacement of chromosomal sequences with the exogenous DNA occurred with precision. If, on the other hand, the target-homologous DNA on one arm of the vector was reduced to below 1 kb in length, the fidelity of recombination was diminished.

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References

    1. Proc Natl Acad Sci U S A. 1989 Sep;86(18):7087-91 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Nov;86(21):8507-11 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Nov;85(22):8583-7 - PubMed
    1. Nature. 1986 Nov 6-12;324(6092):34-8 - PubMed
    1. Science. 1990 Jun 8;248(4960):1227-30 - PubMed