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. 2005 Mar;19(1):80-3.

[Design and rapid evaluation of a TaqMan assay for the detection of influenza A viruses]

[Article in Chinese]
Ji-ming Chen  1 , Zhi-liang Wang, Zeng-chang Pang, Cheng-ying Sun, Ying-xue Sun, Cui-ping Song, Ying Yi
Affiliations
  • PMID: 16201483

[Design and rapid evaluation of a TaqMan assay for the detection of influenza A viruses]

[Article in Chinese]
Ji-ming Chen et al. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2005 Mar.

Abstract

Objective: To design and rapidly evaluate a TaqMan assay for detecting influenza A viruses.

Methods: The probe and the primers of the assay were designed with the software packages of DNA Star and Primer Premier 5.0. Their specificity and conservation were verified through Blast in GenBank and electronic hybridization. The assay's sensitivity was compared with the standard RT-PCR.

Results: The designed primers and probe were confirmed to be very specific and conserved. The assay was 3-27 folds more sensitive than the standard RT-PCR. The RT and PCR steps could be simplified into one step.

Conclusion: The TaqMan Real-time PCR assay is specific, sensitive and easy to perform.

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