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. 2005 Oct;43(10):5055-7.
doi: 10.1128/JCM.43.10.5055-5057.2005.

Real-time reverse transcription PCR for detection and quantitative analysis of equine influenza virus

Michelle Quinlivan  1 , Eugene Dempsey, Fergus Ryan, Sean Arkins, Ann Cullinane
Affiliations

Real-time reverse transcription PCR for detection and quantitative analysis of equine influenza virus

Michelle Quinlivan et al. J Clin Microbiol. 2005 Oct.

Abstract

Equine influenza is a cause of epizootic respiratory disease of the equine. The detection of equine influenza virus using real-time Light Cycler reverse transcription (RT)-PCR technology was evaluated over two influenza seasons with the analysis of 171 samples submitted for viral respiratory disease. Increased sensitivity was found in overall viral detection with this system compared to Directigen Flu A and virus isolation, which were 40% and 23%, respectively, that of the RT-PCR. The assay was also evaluated as a viable replacement for the more traditional methods of quantifying equine influenza virus, 50% egg infectious dose and 50% tissue culture infectious dose. There was a significant positive correlation (P<0.05) between the quantitative RT-PCR and both of these assays.

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Figures

FIG. 1.
FIG. 1.
Mean and standard error of the mean of viral RNA copies/ml (log10) in nasal secretions of four foals from days 2 to 7 postchallenge with equine influenza virus.
See this image and copyright information in PMC

References

    1. Daly, J., T. Blunden, K. Smith, G. Dowd, N. Davis-Poynter, J. Miller, and S. MacRae. 2004. Suspected equine influenza infection in dogs. Office Int. Epizoot. Bull. 2004(2):50.
    1. de Jong, M. D., V. C. Bach, T. Q. Phan, M. H. Vo, T. T. Tran, B. H. Nguyen, M. Beld, T. P. Le, H. K. Truong, V. V. Nguyen, T. H. Tran, Q. H. Do, and J. Farrar. 2005. Fatal avian influenza A (H5N1) in a child presenting with diarrhea followed by coma. N. Engl. J. Med. 352:686-691. - PubMed
    1. Dowdle, W. R., and M. A. Hattwick. 1977. Swine influenza virus infections in humans. J. Infect. Dis. 136(Suppl.):S386-389. - PubMed
    1. Fouchier, R. A., T. M. Bestebroer, S. Herfst, L. Van Der Kemp, G. F. Rimmelzwaan, and A. D. Osterhaus. 2000. Detection of influenza A viruses from different species by PCR amplification of conserved sequences in the matrix gene. J. Clin. Microbiol. 38:4096-4101. - PMC - PubMed
    1. Koopmans, M., R. Fouchier, B. Wilbrink, A. Meijer, G. Natrop, A. D. M. E. Osterhaus, J. E. van Steenbergen, M. du Ry van Beest Holle, M. A. E. Conyn van Spaendonck, and A. Bosman. 2003. Update on human infections with highly pathogenic avian influenza virus A/H7N7 during an outbreak in poultry in the Netherlands. Eurosurveill. Wkly. 7:1-4.

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