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. 1992 Apr;202(1):89-95.
doi: 10.1016/0003-2697(92)90211-o.

Extraction and isolation of mRNA from adult articular cartilage

M E Adams  1 , D Q Huang, L Y Yao, L J Sandell
Affiliations

Extraction and isolation of mRNA from adult articular cartilage

M E Adams et al. Anal Biochem. 1992 Apr.

Abstract

We have developed a method to isolate RNA in high yield from adult articular cartilage. Homogenization of the articular cartilage with a freezer mill, extraction with 4 M guanidinium isothiocyanate/acid-phenol, and ultracentrifugation in cesium trifluoroacetate was found to be an effective and practical method for isolating a high yield of intact RNA from adult canine articular cartilage. The total RNA was suitable for Northern blot analysis. The mRNA that could then be isolated by oligo-dT affinity chromatography was found to be a suitable substrate for in vitro translation, for making a cDNA library, and for PCR amplification.

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