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. 2005 Oct 12:6:246.
doi: 10.1186/1471-2105-6-246.

T.I.M.S: TaqMan Information Management System, tools to organize data flow in a genotyping laboratory

Stéphanie Monnier  1 , David G Cox, Tim Albion, Federico Canzian
Affiliations

T.I.M.S: TaqMan Information Management System, tools to organize data flow in a genotyping laboratory

Stéphanie Monnier et al. BMC Bioinformatics. .

Abstract

Background: Single Nucleotide Polymorphism (SNP) genotyping is a major activity in biomedical research. The Taqman technology is one of the most commonly used approaches. It produces large amounts of data that are difficult to process by hand. Laboratories not equipped with a Laboratory Information Management System (LIMS) need tools to organize the data flow.

Results: We propose a package of Visual Basic programs focused on sample management and on the parsing of input and output TaqMan files. The code is written in Visual Basic, embedded in the Microsoft Office package, and it allows anyone to have access to those tools, without any programming skills and with basic computer requirements.

Conclusion: We have created useful tools focused on management of TaqMan genotyping data, a critical issue in genotyping laboratories without a more sophisticated and expensive system, such as a LIMS.

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Figures

Figure 1
Figure 1
Data flow in a SNP genotyping laboratory. Data flow is divided into management of samples, genotyping assays and genotyping results (dotted outlines). In bold, names of macros described in the text. All operations can be interfaced with a local database storing e.g. details about names of samples in plates, sequences of primers and probes used as genotyping assays, genotyping results.
See this image and copyright information in PMC

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