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. 1992 Jun;58(6):1857-60.
doi: 10.1128/aem.58.6.1857-1860.1992.

API Listeria, a new and promising one-day system to identify Listeria isolates

J Bille  1 , B Catimel, E Bannerman, C Jacquet, M N Yersin, I Caniaux, D Monget, J Rocourt
Affiliations

API Listeria, a new and promising one-day system to identify Listeria isolates

J Bille et al. Appl Environ Microbiol. 1992 Jun.

Abstract

API Listeria is a new 10-test strip for 24-h biochemical identification of Listeria isolates. With this commercial system, 85% of 646 Listeria strains, including atypical isolates selected for this study, were recognized at the species and subspecies level without a complementary test. A new test differentiates Listeria monocytogenes from L. innocua on the basis of the absence of arylamidase from the former. With this system, 97.7% (252 of 258) of the L. monocytogenes strains tested were correctly identified and differentiated from 99.4% (175 of 176) of the L. innocua strains also tested. Gram-positive bacteria other than Listeria spp. gave quite different biochemical patterns. This system considerably reduced the time needed for conventional identification, since results were available within 18 to 24 h.

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