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. 2005 Oct 15:5:15.
doi: 10.1186/1471-2210-5-15.

Curcumin, a diferuloylmethane, attenuates cyclosporine-induced renal dysfunction and oxidative stress in rat kidneys

Naveen Tirkey  1 , Gaganjit Kaur, Garima Vij, Kanwaljit Chopra
Affiliations

Curcumin, a diferuloylmethane, attenuates cyclosporine-induced renal dysfunction and oxidative stress in rat kidneys

Naveen Tirkey et al. BMC Pharmacol. .

Abstract

Background: In India, Curcumin (CMN) is popularly known as "Haldi", and has been well studied due to its economic importance. Traditional Indian medicine claims the use of its powder against biliary disorders, anorexia, coryza, cough, diabetic wounds, hepatic disorder, rheumatism and sinusitis. This study was designed to examine the possible beneficial effect of CMN in preventing the acute renal failure and related oxidative stress caused by chronic administration of cyclosporine (CsA) in rats. CMN was administered concurrently with CsA (20 mg/kg/day s.c) for 21 days. Oxidative stress in kidney tissue homogenates was estimated using thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) content, superoxide dismutase (SOD), and Catalase (CAT). Nitrite levels were estimated in serum and tissue homogenates.

Results: CsA administration for 21 days produced elevated levels of TBARS and marked depletion of renal endogenous antioxidant enzymes and deteriorated the renal function as assessed by increased serum creatinine, Blood Urea Nitrogen (BUN) and decreased creatinine and urea clearance as compared to vehicle treated rats. CMN markedly reduced elevated levels of TBARS, significantly attenuated renal dysfunction increased the levels of antioxidant enzymes in CsA treated rats and normalized the altered renal morphology.

Conclusion: In conclusion our study showed that CMN through its antioxidant activity effectively salvaged CsA nephrotoxicity.

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Figures

Figure 1
Figure 1
Effect of curcumin (CMN) on Cyclosporine-induced lipid peroxidation in rat kidney. Values are expressed mean ± mean. a = Statistical significant at P < 0.05 as compared to control, b = Statistical significant at P < 0.05 as compared to Cyclosporine (CsA).
Figure 2
Figure 2
Effect of curcumin (CMN) on Cyclosporine-induced Reduced Glutathione in rat kidney. Values are expressed mean ± mean. a = Statistical significant at P < 0.05 as compared to control, b = Statistical significant at P < 0.05 as compared to Cyclosporine (CsA).
Figure 3
Figure 3
Effect of curcumin (CMN) on Cyclosporine-induced SOD levels in rat kidney. Values are expressed mean ± mean. a = Statistical significant at P < 0.05 as compared to control, b = Statistical significant at P < 0.05 as compared to Cyclosporine (CsA).
Figure 4
Figure 4
Effect of curcumin (CMN) on Cyclosporine-induced catalase levels in rat kidney. Values are expressed mean ± mean. a = Statistical significant at P < 0.05 as compared to control, b = Statistical significant at P < 0.05 as compared to Cyclosporine (CsA).
Figure 5
Figure 5
(A) Hematoxylin and Eosin-stained sections of Normal rat kidneys. (B) Kidney section of CsA treated rats showing tubular brush-border loss, interstitial oedema, Necrosis of epithelium and Hyaline Casts. (C) Kidney Section of CMN (15 mg/kg p.o) + CsA treated rats showing prevention of CsA induced alterations. (D) Kidney section of CMN (15 mg/kg p.o.) treated rats showing almost normal morphology.
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