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. 2005 Nov;12(11):952-7.
doi: 10.1038/nsmb1005.

Dual modes of RNA-silencing suppression by Flock House virus protein B2

Jeffrey A Chao  1 , June Hyung Lee, Brian R Chapados, Erik W Debler, Anette Schneemann, James R Williamson
Affiliations

Dual modes of RNA-silencing suppression by Flock House virus protein B2

Jeffrey A Chao et al. Nat Struct Mol Biol. 2005 Nov.

Abstract

As a counter-defense against antiviral RNA silencing during infection, the insect Flock House virus (FHV) expresses the silencing suppressor protein B2. Biochemical experiments show that B2 binds to double-stranded RNA (dsRNA) without regard to length and inhibits cleavage of dsRNA by Dicer in vitro. A cocrystal structure reveals that a B2 dimer forms a four-helix bundle that binds to one face of an A-form RNA duplex independently of sequence. These results suggest that B2 blocks both cleavage of the FHV genome by Dicer and incorporation of FHV small interfering RNAs into the RNA-induced silencing complex.

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