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Clinical Trial
. 2005 Sep;11(9):1461-3.
doi: 10.3201/eid1109.041236.

Multidrug-resistant tuberculosis detection, Latvia

Girts Skenders  1 , Alicia M Fry, Inga Prokopovica, Silvija Greckoseja, Lonija Broka, Beverly Metchock, Timothy H Holtz, Charles D Wells, Vaira Leimane
Affiliations
Clinical Trial

Multidrug-resistant tuberculosis detection, Latvia

Girts Skenders et al. Emerg Infect Dis. 2005 Sep.

Abstract

To improve multidrug-resistant tuberculosis (MDR-TB) detection, we successfully introduced the rpoB gene mutation line probe assay into the national laboratory in Latvia, a country with epidemic MDR-TB. The assay detected rifampin resistance with 91% sensitivity and 96% specificity within 1 to 5 days (vs. 12-47 days for BACTEC).

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References

    1. Kim BJ, Lee KH, Park BN, Kim SJ, Park EM, Park YG, et al. Detection of rifampin-resistant Mycobacterium tuberculosis in sputa by nested PCR-linked single-strand conformation polymorphism and DNA sequencing. J Clin Microbiol. 2001;39:2610–7. 10.1128/JCM.39.7.2610-2617.2001 - DOI - PMC - PubMed
    1. Patnaik M, Liegmann K, Peter JB. Rapid detection of smear-negative Mycobacterium tuberculosis by PCR and sequencing for rifampin resistance with DNA extracted directly from slides. J Clin Microbiol. 2001;39:51–2. 10.1128/JCM.39.1.51-52.2001 - DOI - PMC - PubMed
    1. Garcia L, Alonso-Sanz M, Rebollo MJ, Tercero JC, Chaves F. Mutations in the rpoB gene of rifampin-resistant Mycobacterium tuberculosis isolates in Spain and their rapid detection by PCR-enzyme-linked immunosorbent assay. J Clin Microbiol. 2001;39:1813–8. 10.1128/JCM.39.5.1813-1818.2001 - DOI - PMC - PubMed
    1. Caviedes L, Lee TS, Gilman RH, Sheen P, Spellman E, Lee EH, et al. Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures. The Tuberculosis Working Group in Peru. J Clin Microbiol. 2000;38:1203–8. - PMC - PubMed
    1. Watterson SA, Wilson SM, Yates MD, Drobniewski FA. Comparison of three molecular assays for rapid detection of rifampin resistance in Mycobacterium tuberculosis. J Clin Microbiol. 1998;36:1969–73. - PMC - PubMed

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