Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 May;19(2):309-11.
doi: 10.1007/BF00027352.

Combination of in vitro capping and ribonuclease protection improves the detection of transcription start sites in chloroplasts

A Vera  1 , M Sugiura
Affiliations

Combination of in vitro capping and ribonuclease protection improves the detection of transcription start sites in chloroplasts

A Vera et al. Plant Mol Biol. 1992 May.

Abstract

A primary transcript from the chloroplast rpl32 gene was labelled at its 5' end using a capping enzyme and [alpha-32P]GTP followed by hybridization to a cold RNA probe. A RNase protection assay gave a clear protected band and its initiation site of transcription could thus be estimated, which had not been possible by using DNA probes. The combination of in vitro capping and RNase protection is an excellent method for mapping transcription initiation sites on the chloroplast genome and shows a high improvement relative to the DNA-employing strategies.

PubMed Disclaimer

References

    1. Mol Cell Biol. 1985 Oct;5(10):2733-45 - PubMed
    1. Methods Enzymol. 1989;180:334-47 - PubMed
    1. Curr Genet. 1990 Jan;17(1):65-71 - PubMed
    1. Curr Genet. 1991 Aug;20(3):259-64 - PubMed
    1. FEBS Lett. 1990 Dec 10;276(1-2):88-90 - PubMed

LinkOut - more resources