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. 2002;94(4):362-4.
doi: 10.1263/jbb.94.362.

Detection of protein-protein interaction by bioluminescence resonance energy transfer from firefly luciferase to red fluorescent protein

Ryoichi Arai  1 , Hideyuki Nakagawa, Atsushi Kitayama, Hiroshi Ueda, Teruyuki Nagamune
Affiliations

Detection of protein-protein interaction by bioluminescence resonance energy transfer from firefly luciferase to red fluorescent protein

Ryoichi Arai et al. J Biosci Bioeng. 2002.

Abstract

We describe a novel homogeneous protein-protein interaction assay based on bioluminescence resonance energy transfer (BRET) from mutant firefly luciferase to red fluorescent protein (DsRed). The association of glutathione S-transferase (GST)-luciferase and protein G-DsRed, which was driven by the addition of anti-GST antibody, was successfully monitored by BRET.

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