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. 2003;96(6):581-4.
doi: 10.1016/S1389-1723(04)70154-1.

Stereoselective biotransformation of limonene and limonene oxide by cyanobacterium, Synechococcus sp. PCC 7942

Hiroki Hamada  1 , Yoko Kondo, Kohji Ishihara, Nobuyoshi Nakajima, Hatsuyuki Hamada, Ryo Kurihara, Toshifumi Hirata
Affiliations

Stereoselective biotransformation of limonene and limonene oxide by cyanobacterium, Synechococcus sp. PCC 7942

Hiroki Hamada et al. J Biosci Bioeng. 2003.

Abstract

The biotransformation of limonene and limonene oxide by the cyanobacterium, Synechococcus sp. PCC 7942, was investigated. (S)-(+)-Limonene was hydroxylated stereo- and regioselectively at its allylic position of the endocyclic C=C double bond by the cyanobacterial cells to its corresponding alcohol. The cells also showed the ability for the enantio- and stereoselective cleavage of the epoxide group of (1S,2R,4R)-limonene oxide to give (1S,2S,4R)-limonene-1,2-diol. The repetitive production of carveol from limonene was achieved using Ca2+-alginate-immobilized cyanobacterial cells.

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