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Review
. 2005 Nov;73(11):7089-98.
doi: 10.1128/IAI.73.11.7089-7098.2005.

Expression library immunization: a road map for discovery of vaccines against infectious diseases

Adel M Talaat  1 , Katherine Stemke-Hale
Affiliations
Review

Expression library immunization: a road map for discovery of vaccines against infectious diseases

Adel M Talaat et al. Infect Immun. 2005 Nov.
No abstract available

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Figures

FIG. 1.
FIG. 1.
An outline of ELI protocol. The entire genome of a pathogen is fractioned into small fragments before cloning into a mammalian expression vector creating a library that would express all the ORFs of a pathogen. Groups of animals can be immunized with separate plasmid pools followed by a pathogen challenge using a suitable route of infection. Plasmids from a protected mouse group(s) will be further divided into smaller pools and retested in other mice groups to identify single protective antigens.
FIG. 2.
FIG. 2.
Microarrays and vaccine development. Gene lists generated from different analyses of microarray hybridizations (such as hierarchical clustering) will be individually cloned into a mammalian expression system before further screening in an ELI protocol as outlined in Fig. 1.
See this image and copyright information in PMC

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