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. 2005 Dec 1;253(1):155-61.
doi: 10.1016/j.femsle.2005.09.032. Epub 2005 Oct 7.

Loop-mediated isothermal amplification for the rapid detection of Salmonella

Yukiko Hara-Kudo  1 , Manabu Yoshino, Tadashi Kojima, Masanari Ikedo
Affiliations

Loop-mediated isothermal amplification for the rapid detection of Salmonella

Yukiko Hara-Kudo et al. FEMS Microbiol Lett. .

Abstract

Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity of the LAMP assay was found to be >2.2 cfu/test tube using nine serotypes. The specificity was similar to that of a PCR assay, but the sensitivity of LAMP was greater. Both fluorescence and turbidity were able to detect the products in the LAMP assay. S. enteritidis in a liquid egg sample artificially inoculated with the organism was detected by the LAMP assay at 2.8 cfu/test tube, although negative by PCR assay. These results indicate that the LAMP assay is a rapid, specific and sensitive detection method for Salmonella.

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