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. 1992 Jul;174(13):4513-6.
doi: 10.1128/jb.174.13.4513-4516.1992.

Genetic and morphological characterization of an Escherichia coli chromosome segregation mutant

P S Stewart  1 , R D'Ari
Affiliations

Genetic and morphological characterization of an Escherichia coli chromosome segregation mutant

P S Stewart et al. J Bacteriol. 1992 Jul.

Abstract

The temperature-sensitive nucleoid segregation mutant of Escherichia coli, PAT32, formerly described as a parA mutant, has been shown to carry a mutation near 66 min on the genetic map. Fine mapping with phages from the collection of Kohara et al. is consistent with its being a parC allele. Observation by fluorescence microscopy revealed the formation, at a nonpermissive temperature, of filaments containing one or two large nucleoids and of normal-size anucleate cells. There was also a significant loss of viability.

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