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. 1992 Jul;174(14):4838-41.
doi: 10.1128/jb.174.14.4838-4841.1992.

Isolation, characterization, and physiological role of the pyruvate dehydrogenase complex and alpha-acetolactate synthase of Lactococcus lactis subsp. lactis bv. diacetylactis

J L Snoep  1 , M J Teixeira de Mattos, M J Starrenburg, J Hugenholtz
Affiliations

Isolation, characterization, and physiological role of the pyruvate dehydrogenase complex and alpha-acetolactate synthase of Lactococcus lactis subsp. lactis bv. diacetylactis

J L Snoep et al. J Bacteriol. 1992 Jul.

Abstract

The pyruvate dehydrogenase complex of Lactococcus lactis subsp. lactis bv. diacetylactis has a specific activity of 6.6 U/mg and a Km of 1 mM for pyruvate. The specific activities of E2 and E3 in the complex are 30 and 0.36 U/mg, respectively. The complex is very sensitive to NADH inhibition and consists of four subunits: E1 alpha (44 kDa), E1 beta (35 kDa), E2 (73 kDa), and E3 (60 kDa). The L. lactis alpha-acetolactate synthase has a specific activity of 103 U/mg and a Km of 50 mM for pyruvate. Thiamine pyrophosphate (Km = 3.2 microM) and divalent cations are essential for activity. The native enzyme measures 172 kDa and consists of 62-kDa monomers. The role of both enzymes in product formation is discussed in view of NADH inhibition and competition for pyruvate.

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References

    1. J Bacteriol. 1975 Mar;121(3):917-22 - PubMed
    1. Plant Physiol. 1990 Jul;93(3):1027-31 - PubMed
    1. Eur J Biochem. 1992 Jan 15;203(1-2):245-50 - PubMed
    1. Arch Microbiol. 1990;154(1):50-5 - PubMed
    1. FEBS Lett. 1989 Sep 11;255(1):77-82 - PubMed

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