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. 2005 Nov 2;127(43):14954-5.
doi: 10.1021/ja0530319.

A strategy for the development of small-molecule-based sensors that strongly fluoresce when bound to a specific RNA

Brian A Sparano  1 , Kazunori Koide
Affiliations

A strategy for the development of small-molecule-based sensors that strongly fluoresce when bound to a specific RNA

Brian A Sparano et al. J Am Chem Soc. .

Abstract

We report a broadly applicable approach for the development of small-molecule-based RNA sensors. Our photoinduced electron transfer (PET) sensor consists of a fluorescein derivative as the fluorophore and two aniline derivatives as electron donors (quenchers). The isolation of electron-donor-binding RNA by in vitro selection (also known as SELEX) yielded an RNA aptamer that could increase the fluorescence intensity of the sensor by 13-fold. This result shows that RNA-electron-donor interactions can be used to develop modular RNA chemosensors.

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