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. 2005 Nov;49(11):4760-2.
doi: 10.1128/AAC.49.11.4760-4762.2005.

Kinetics study of KPC-3, a plasmid-encoded class A carbapenem-hydrolyzing beta-lactamase

Jimena Alba  1 , Yoshikazu Ishii, Kenneth Thomson, Ellen Smith Moland, Keizo Yamaguchi
Affiliations

Kinetics study of KPC-3, a plasmid-encoded class A carbapenem-hydrolyzing beta-lactamase

Jimena Alba et al. Antimicrob Agents Chemother. 2005 Nov.

Abstract

The kinetic activity of KPC-3, a plasmid-encoded class A carbapenemase, was studied. It hydrolyzed penicillins, cephalosporins, carbapenems, and even sulbactam. The best substrate was cephalothin (k(cat/K)m = 3.48 microM(-1) s(-1)). The efficiency of the enzyme was similar for imipenem and meropenem (k(cat)/K(m), 1.4 and 1.94 microM(-1) s(-1), respectively).

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