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. 2005 Dec 1;41(11):1677-80.
doi: 10.1086/497595. Epub 2005 Oct 28.

Prolonged incubation and extensive subculturing do not increase recovery of clinically significant microorganisms from standard automated blood cultures

Ellen Jo Baron  1 , John D Scott, Lucy S Tompkins
Affiliations

Prolonged incubation and extensive subculturing do not increase recovery of clinically significant microorganisms from standard automated blood cultures

Ellen Jo Baron et al. Clin Infect Dis. .

Abstract

An extensive blood culture protocol, including prolonged incubation of cultures, for 215 patients believed to have had endocarditis yielded only 3 clinically relevant results. Twenty-four Haemophilus, Actinobacillus, Cardiobacterium, Eikenella, and Kingella (i.e., HACEK) organisms were recovered from standard 5-day blood cultures during the same time period. Specialized methods and not extended incubation times are recommended for recovery of fastidious agents of septicemia.

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