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. 1992 Jul;19(4):699-703.
doi: 10.1007/BF00026796.

A barley cDNA clone encoding a type III chlorophyll a/b-binding polypeptide of the light-harvesting complex II

J Brandt  1 , V S Nielsen, H Thordal-Christensen, D J Simpson, J S Okkels
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A barley cDNA clone encoding a type III chlorophyll a/b-binding polypeptide of the light-harvesting complex II

J Brandt et al. Plant Mol Biol. 1992 Jul.

Abstract

The nucleotide sequence of a leaf cDNA clone encoding a Type III chlorophyll a/b-binding (CAB) protein of light-harvesting complex II (LHCII) in barley is reported. Sequence comparisons and results from in vitro import into chloroplasts demonstrate that the cDNA clone encodes a functional transit peptide of 45 amino acid residues and a mature polypeptide of 223 residues with a predicted molecular mass of 24.3 kDa. After insertion into thylakoids, the mature protein is resistant to protease attack. Hybridization analysis using a gene-specific probe shows that the gene is expressed in dark-grown seedlings and that the amount of mRNA increases during illumination.

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References

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