Role of cultivation media in the development of yeast strains for large scale industrial use

Abstract

The composition of cultivation media in relation to strain development for industrial application is reviewed. Heterologous protein production and pentose utilization by Saccharomyces cerevisiae are used to illustrate the influence of media composition at different stages of strain construction and strain development. The effects of complex, defined and industrial media are compared. Auxotrophic strains and strain stability are discussed. Media for heterologous protein production and for bulk bio-commodity production are summarized.

Figure 1

Principle of Evolutionary Engineering [18-20].

Figure 2

Principe of Metabolic Engineering (adapted from [23]).

Figure 3

Aerobic growth of baker's yeast in YP medium buffered with 50 mM Phthalate. With glucose -◊- (μmax = 0.45), Without glucose -×- (μmax = 0.29)

Figure 4

Xylose utilisation and product formation during oxygen-limited cultivation of TMB 3400 [29] in YP and YNB media. Xylose YP-●-, Xylose YNB -○-, Ethanol YP -▴-, Ethanol YNB -△-, OD₆₂₀ YP -■-, OD₆₂₀ YNB -□- Sugars and products were analyzed with HPLC (Bio-Rad, Aminex 87-H column).

Figure 5

a-d. Influence of 50 mM buffer on aerobic growth of TMB 3001 [62] in YP and DM media. a, c: glucose 20 g/l in YP, b, d: glucose 20 g/l in DM. Non buffered -○-, Phthalate -□-, Phosphate -△-, Citrate-Phosphate -×-, Citrate -◊-