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doi: 10.1016/j.cell.2005.11.006.
RNAi: RISC gets loaded
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- PMID: 16286001
- DOI: 10.1016/j.cell.2005.11.006
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Comment
RNAi: RISC gets loaded
Cell.
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Free article
Abstract
When an siRNA or miRNA proceeds through the RNA-induced silencing complex assembly pathway, only one of the two approximately 21-nucleotide RNA strands survives in the final, active complex. In this issue of Cell, Matranga et al. (2005) and Rand et al. (2005) reveal the fate of the rejected passenger siRNA strand. Additionally, Gregory et al. (2005) define a heterotrimeric complex from humans that appears to execute dsRNA loading, strand selection, and target mRNA cleavage activities.
Comment on
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Argonaute2 cleaves the anti-guide strand of siRNA during RISC activation.Cell. 2005 Nov 18;123(4):621-9. doi: 10.1016/j.cell.2005.10.020. Epub 2005 Nov 3. Cell. 2005. PMID: 16271385
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Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes.Cell. 2005 Nov 18;123(4):607-20. doi: 10.1016/j.cell.2005.08.044. Epub 2005 Nov 3. Cell. 2005. PMID: 16271386
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Human RISC couples microRNA biogenesis and posttranscriptional gene silencing.Cell. 2005 Nov 18;123(4):631-40. doi: 10.1016/j.cell.2005.10.022. Epub 2005 Nov 3. Cell. 2005. PMID: 16271387
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