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. 1992 Aug;174(15):5171-5.
doi: 10.1128/jb.174.15.5171-5175.1992.

A general method for cloning recA genes of gram-positive bacteria by polymerase chain reaction

P Duwat  1 , S D Ehrlich, A Gruss
Affiliations

A general method for cloning recA genes of gram-positive bacteria by polymerase chain reaction

P Duwat et al. J Bacteriol. 1992 Aug.

Abstract

An internal fragment of the recA gene from eight gram-positive organisms has been amplified by using degenerate primers in a polymerase chain reaction. The internal 348- or 360-bp recA DNA segments from Bacillus subtilis, Clostridium acetobutylicum, Lactobacillus bulgaricus, Lactobacillus helveticus, Leuconostoc mesanteroides, Listeria monocytogenes, Staphylococcus aureus, and Streptococcus salivarus subsp. thermophilus were amplified, cloned, and sequenced. The G + C contents of the DNA from these species range from 28 to 52%. The sequences of the bacterial recA genes show strong relatedness. This method is particularly useful for the recovery of the recA genes of gram-positive bacteria and avoids the difficulties of using a genetic complementation test for cloning.

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References

    1. J Bacteriol. 1992 Apr;174(8):2729-32 - PubMed
    1. Nature. 1992 Jan 23;355(6358):374-6 - PubMed
    1. J Bacteriol. 1992 Feb;174(3):778-84 - PubMed
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