Detection of adenoviruses in stools from healthy persons and patients with diarrhea by two-step polymerase chain reaction
- PMID: 1629713
- DOI: 10.1002/jmv.1890370214
Detection of adenoviruses in stools from healthy persons and patients with diarrhea by two-step polymerase chain reaction
Abstract
The use of the polymerase chain reaction (PCR) for detection of human adenoviruses in diluted stool samples was investigated. Two sets of nested primers, including primers specific for the hexon-coding region and for the E1B region of enteric adenoviruses (EAd), were assessed by two-step amplification. The primers constitute two different PCR systems designed for the detection of adenoviruses belonging to all six subgenera (A-F), and the two EAds Ad40 and Ad41, respectively. In a two-step PCR mediated amplification a single virus particle was detected when the two sets of general hexon primers or EAd specific primers were used. Earlier results from PCR detection of adenoviruses in stool from children suffering from diarrhea gave indications that adenovirus particles are commonly shed in stools without being identified as the cause of illness [Allard et al.: Journal of Clinical Microbiology 28:2659-2667, 1990]. Therefore, the general and the EAd specific PCR assays were assessed on 150 stool specimens from three groups including 50 healthy children, 50 healthy adults, and 50 adults suffering from diarrhea. When the two sets of general hexon primers were used, 25 of the 50 specimens from the healthy children (mean age 21 months) were found positive by two-step PCR amplification. Nine of the 50 specimens from the healthy adults (mean age 32 years) were found positive whereas 12 of the 50 specimens from sick adults (mean age 31 years) gave amplification products, using the two sets of general hexon primers in a nested fashion. None of the 150 specimens were found to be positive by two-step PCR amplification using the two sets of EAd-specific primers.(ABSTRACT TRUNCATED AT 250 WORDS)
Similar articles
-
Polymerase chain reaction for detection of adenoviruses in stool samples.J Clin Microbiol. 1990 Dec;28(12):2659-67. doi: 10.1128/jcm.28.12.2659-2667.1990. J Clin Microbiol. 1990. PMID: 2279998 Free PMC article.
-
Rapid detection of enteric adenoviruses by means of the polymerase chain reaction.J Infect. 1993 Nov;27(3):271-5. doi: 10.1016/0163-4453(93)92097-g. J Infect. 1993. PMID: 8308319
-
Detection and typing of subgroup F adenoviruses using the polymerase chain reaction.J Virol Methods. 1996 May;59(1-2):73-82. doi: 10.1016/0166-0934(96)02015-0. J Virol Methods. 1996. PMID: 8793832
-
PCR-based detection and typing of human adenoviruses in clinical samples.Res Virol. 1997 May-Jun;148(3):225-31. doi: 10.1016/s0923-2516(97)83992-1. Res Virol. 1997. PMID: 9201813
-
Enteric adenoviruses.Baillieres Clin Gastroenterol. 1990 Sep;4(3):627-42. doi: 10.1016/0950-3528(90)90053-j. Baillieres Clin Gastroenterol. 1990. PMID: 1962727 Review.
Cited by
-
Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays.Virol J. 2013 May 28;10:166. doi: 10.1186/1743-422X-10-166. Virol J. 2013. PMID: 23714224 Free PMC article.
-
Viruses causing gastroenteritis.Clin Microbiol Infect. 2003 Apr;9(4):247-62. doi: 10.1046/j.1469-0691.2003.00560.x. Clin Microbiol Infect. 2003. PMID: 12667234 Free PMC article. Review.
-
Respiratory viral infections in patients with chronic, obstructive pulmonary disease.J Infect. 2005 May;50(4):322-30. doi: 10.1016/j.jinf.2004.07.011. J Infect. 2005. PMID: 15845430 Free PMC article.
-
Environmental factors influencing human viral pathogens and their potential indicator organisms in the blue mussel, Mytilus edulis: the first Scandinavian report.Appl Environ Microbiol. 2002 Sep;68(9):4523-33. doi: 10.1128/AEM.68.9.4523-4533.2002. Appl Environ Microbiol. 2002. PMID: 12200309 Free PMC article.
-
Prevalence and molecular characterisation of human adenovirus in diarrhoeic children in Tanzania; a case control study.BMC Infect Dis. 2014 Dec 12;14:666. doi: 10.1186/s12879-014-0666-1. BMC Infect Dis. 2014. PMID: 25495029 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical