Early events in yeast mitochondrial protein targeting

Abstract

Protein import into mitochondria involves a number of complex steps occurring in the cytosol, on the mitochondrial surface, and inside the organelle. Once an initial interaction between mitochondrial proteins and their specific receptors occurs, the proteins are transported into the organelle in a series of reactions involving (in the case of a protein to be translocated into the mitochondrial matrix) the mitochondrial membrane potential, ATP hydrolysis and an undetermined number of membrane components. Inside the organelle, mitochondrial proteins are processed and sorted to their final intramitochondrial destinations. The earliest steps in the import process take place in the cytosol and include the synthesis of the mitochondrial proteins themselves, their interaction with cytosolic factors, and perhaps the establishment of cotranslational import complexes on the mitochondrial surface. These early events are important because it is during this phase that the system as a whole is most sensitive to cytosolic conditions that may exert control over the entire import process.